Cellulose is an important component of plant cell walls. It is straight chain polymer composed of glucose molecules with β-1,4- beta glycosidic bond at the weight of 1/2 ~ 1/3 of total plant. It is the largest renewable substance in nature, and its degradation is the central link of the carbon cycle in nature.
Methods for producing cellulases include solid fermentation and liquid fermentation. Production raw materials are wheat bran, straw powder, waste paper, corn flour and salt. In the presence of cellulase, cellulose can easily be decomposed into small molecules for further fermentation of acetic acid, alcohol and lactic acid.
Characteristics of Cellulase
Cellulase is a generic term which can degrade cellulose into multicomponent enzymes of glucose.
1. Composition of cellulase
Cellulase is generally classified into three groups:
Endoglucanase
Exoglucanase
Β-glucosidase
2. Properties of cellulase
There are wide sources of cellulase. The enzymatic properties of cellulase produced by different sources and different fermentation methods are different. The reaction temperature for cellulase is 30 to 45 ℃, and the optimal reaction temperature is 35 to 37 ℃. In addition, cellulase has high thermal stability. Generally, the optimal temperature is 40 to 65 ℃, and stable temperature range is 50 to 70 ℃. However, the thermal stability of each enzyme is also different, and it is affected by PH.
3. Effect mode of Cellulase
Many researchers have put forward many different views on the process of cellulase converting cellulose into glucose. At present, the generally accepted theory is: firstly, endoglucanase acts on the non-crystalline region of micro-fiber, making it expose many ends for exonuclease action. Cellobiohydrolase is decomposed from non-reductive ends in order to produce cellobiose; then, part of the degradated cellulose further decompose cellobiose or cellotriose by endoglucanase and exonuclease; finally Β-glucosidase breaks it down glucose.
Application of cellulase in food fermentation
Soy sauce brewing
Table vinegar Brewing
Alcohol production
Beer production
Aginomoto production
Application of cellulase in food processing
Fruit and vegetable processing
Soybean and instant noodles processing
Instant tea production
Beverage industry
Beyond above applications, cellulases can be used in many other fields, such as baking enzymes, acid cellulase for textile, native cellulase from talaromyces emersonii, etc. At Creative Enzymes, we provides a full rang of cellulase products in various applications. Contact us for more information.
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